Fig. 5. Inhibition of HIF1α rescued the effect of WWOX loss.

a Digoxin experiment plan, DEN was IP injected to control and Wwox^ΔHep mice at the age of 14 days. Six-month later mice were started to be treated with digoxin (1 mg/kg) or vehicle control (saline) 3-times a week for an additional 8-months. b Representative images of saline-treated Wwox^ΔHep (cKO) mice versus digoxin-treated Wwox^ΔHep (cKO) mice livers and liver sections c Tumor load of saline-treated Wwox^ΔHep mice versus digoxin-treated Wwox^ΔHep mice livers as assessed by liver weight at the age of 14 months d mRNA expression levels of HIF1α glycolytic target genes in saline-treated Wwox^ΔHep (cKO) mice versus digoxin-treated Wwox^ΔHep (cKO) mice at the age of 14 months, (n = 3 for each group). e Χ² analysis of macroscopic tumor incidence in saline-treated control mice versus digoxin-treated control mice at the age of 14 months. f mRNA expression levels of HIF1α glycolytic target genes in saline-treated control mice versus digoxin-treated control mice at the age of 14 months, (n = 3 for each group). * P value < 0.05, ** P value < 0.01, *** P value < 0.001. Error bars indicate ± SEM