Fig. 6. ACTL6A physically interacts with YAP and inhibits protein degradation partially by β-TrCP.

a, b Western blot analysis of co-precipitating proteins in IPs performed using anti-YAP or -ACTL6A antibody on lysates prepared from U251 and U87MG cells. c The design of YAP depletion constructs is based on known functional domain. Interactions were analyzed by IP assay. d Western blot analysis of YAP IPs performed with lysates prepared from cells transfected with increasing amounts of Flag-ACTL6A. e, f Western blot analysis of YAP IPs performed with U251-NC and -sh-ACTL6A or U87MG-NC and -ACTL6A cells treated with MG132 (20 µM) for 8 h. g The design of YAP mutant constructs. Western blot analysis of YAP mutant protein levels plus or minus Flag-ACTL6A. Student’s t-test: n.s. = not significant, **P < 0.01