Fig. 3. Low expression of Dicer1 in MM-MSCs and myeloma cells induce cell senescence in HC-MSC.

a The mRNA expression of Dicer1 in expanded MM-MSC (n = 46) and HC-MSC (n = 18). b The expression of Dicer1 in primary MM-MSC (n = 18) and primary HC-MSC (n = 10). c, d Protein levels of Dicer1 in MM-MSC (n = 4) and HC-MSC (n = 4). e, f HC-MSC (n = 4) were co-cultured with HMCLs: NCI-H929, OPM-2, and KMS-12-BM (e), or with primary CD138⁺ MM tumors cells from three MM patients (f) for 24 h. Relative mRNA expression levels of p21, Dicer1, miR-93, and miR-20a in MSC after co-cultured with Myeloma cells. The results are expressed as means ± SD. Compared with controls, the significance was set as *p ≤ 0.05; **p ≤ 0.01