Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 3;9(5):512. doi: 10.1038/s41419-018-0545-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a–d The expressions of Dicer1 mRNA and protein were dramatically increased after adenovirus transfection. And the protein expression of p21 decreased. e Representative micrographs after SA-β-gal staining of control MSC (MM-MSC without transfection), AD-Dicer1 MSC (MM-MSC transfected with Dicer1 adenovirus lentiviruses) and AD-GFP (MM-MSC transfected with control lentiviruses) (100× magnification). f The percentages of SA-β-gal-positive cells. g Cell cycle analysis of Dicer1-KD MSC by flow cytometric analysis. Dicer1 AD caused an increasing proportion of cells in the S phase and a decrease of those in the G1 phase. h MM-MSCs transfected with AD-Dicer1 proliferate more quickly than either MM-MSCs or MM-MSCs transfected with AD-GFP. i Typical images after Alizarin Red S staining on day 21 of osteogenic differentiation. j Relative calcium production (OD 572 nm) by AD-Dicer1 MSC, was significantly higher after 21 days of differentiation as compared with controls. k The ALP activity of AD-Dicer1 MSC was significantly increased after 3 days osteogenic differentiation. l, m Relative RUNX2 and ALP mRNA expression levels. The results are expressed as means ± SD. The average of three replicates is displayed. Compared with controls, the significance was set as *p ≤ 0.05, ** p ≤ 0.01