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. Author manuscript; available in PMC: 2019 Apr 17.
Published in final edited form as: Biochemistry. 2018 Apr 3;57(15):2238–2244. doi: 10.1021/acs.biochem.8b00279

Figure 1.

Figure 1

31P NMR spectra of the products in the reaction catalyzed by Cj1415. (A) Cj1415 (5 μM) was mixed with 5.0 mM MgATP and 5.0 mM CDP-NH2 for 60 minutes at pH 8.0 before the reaction was quenched with 15 mM EDTA. (B) Cj1415 (5 μM) was mixed with 5.0 mM MgATP and 5.0 mM 2′-deoxy-CDP-NH2 for 60 minutes before the reaction was quenched with 15 mM EDTA. The insets show the 1H-coupled spectra for the resonances at 4.1 ppm (A) and 3.85 ppm (B). Additional details are available in the text.