. Author manuscript; available in PMC: 2019 Apr 17.

Published in final edited form as: Biochemistry. 2018 Apr 3;57(15):2238–2244. doi: 10.1021/acs.biochem.8b00279

Table 1.

Kinetic Constants of Cj1415 at 25 °C, pH 8.0

substrate		k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (M⁻¹ s⁻¹)
CDP-NH₂	(1)	2.2 ± 0.3	0.11 ± 0.01	20000 ± 3300
dCDP-NH₂	(2)	2.2 ± 0.2	0.39 ± 0.07	5600 ± 1100
CDP	(3)	0.64 ± 0.04	4.2 ± 0.7	150 ± 30
CDP-OMe	(4)	0.65 ± 0.06	1.4 ± 0.2	480 ± 90
CDP-Me	(5)	1.4 ± 0.1	0.8 ± 0.2	1700 ± 400
UDP-NH₂	(6)	0.023 ± 0.002	21 ± 3	1.1 ± 0.2
ADP-NH₂	(7)	0.08 ± 0.01	5.6 ± 0.8	14 ± 3
APS	(8)	< 0.01
CDP-NH₂^a	(1)	1.6 ± 0.2	4.4 ± 0.4	360 ± 60
ATP		2.2 ± 0.3	1.5 ± 0.1	1500 ± 200
ATP^a		1.6 ± 0.2	0.7 ± 0.1	2300 ± 500

S85A mutant of Cj1415