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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Biochemistry. 2017 Oct 24;56(46):6079–6082. doi: 10.1021/acs.biochem.7b00905

Figure 2.

Figure 2

31P NMR spectra of nucleotide standards and enzyme-catalyzed reaction products formed in 100 mM HEPES, pH 8.0 at 30 °C with an incubation time of 90 minutes before being quenched with 10 mM EDTA. (A) Control sample containing 5.0 mM CTP, 5.0 mM MgCl2, and 6.0 mM L-glutamine phosphate. (B) 5.0 mM CTP, 5.0 mM MgCl2, 6.0 mM L-glutamine phosphate, 20 μM Cj1416, and 20 μM Cj1417. (C) Control sample of 5.0 mM CDP phosphoramidate.