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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Biochemistry. 2017 Oct 24;56(46):6079–6082. doi: 10.1021/acs.biochem.7b00905

Figure 3.

Figure 3

31P NMR spectra of nucleotide standards and enzyme-catalyzed reaction products formed in 100 mM HEPES, pH 8.0 at 25 °C with an incubation time of 90 minutes before being quenched with 10 mM EDTA. (A) 5.0 mM CTP, 5.0 mM MgCl2, 5 unit/mL of pyrophosphatase, and 20 μM Cj1416. (B). 20 μM Cj1417 was added to the reaction mixture shown in Figure 3A and allowed to reaction for an additional 90 minutes. (C). 20 μM Cj1417 and 5.0 mM glutamine phosphate. (D). Control sample of 5.0 mM phosphoramidate.