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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Biochemistry. 2017 Jul 18;56(30):3900–3912. doi: 10.1021/acs.biochem.7b00024

Figure 1.

Figure 1

Electrophoresis analysis of H-rich and L-rich ferritins. (A) SDS–CGE electropherograms of the Beckman test mixture and the recombinant apo-heteropolymers of human H-rich and L-rich ferritins. The H-rich and L-rich samples were 0.5 mg each and prepared as described elsewhere.25–27 The instrument conditions are specified in Materials and Methods. (B) Electrophoretic analysis of ferritin heteropolymers using 15% SDS–PAGE. The faint bands in the bottom portion of the gel are due to peptide degradation products commonly seen with ferritins. (C) A 7.5% nondenaturing PAGE gel demonstrating the homogeneity of the L-rich and H-rich ferritins and mobilities related to the H- and L-subunit contents. Arrows point to positions of the L- and H-subunit 24-mer homopolymers, designated HuLF and HuHF, respectively. MWM denotes molecular weight markers.