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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Biochemistry. 2017 Jul 18;56(30):3900–3912. doi: 10.1021/acs.biochem.7b00024

Figure 2.

Figure 2

Aerobic spectrophotometric titration of apo H-rich ferritin with Fe(II). Conditions: 2 μM apoprotein in 50 mM Mops and 50 mM NaCl at pH 7.0 and 25 °C, 8 mM stock Fe(II) solution at pH 2.0. Each data point corresponds to the injection of 8 Fe(II) atoms/protein shell [2 μL of Fe(II) added to the 1.0 mL UV cell].