Table 1. Cross-validation of detection of Borrelia spp. by quantitative PCR and ELISA in blood samples from 310 patients with suspected borreliosis, Yekaterinburg, Russia, 2010 and 2015*.
| Quantitative PCR for 16S rRNA gene |
No. patients |
No. ELISA positive |
No. ELISA negative |
ND |
| With erythema migrans | ||||
| Borrelia burgdorferi sensu lato | 31 | 30 | 1 | 0 |
| B. miyamotoi | 5 | 5 | 0 | 0 |
| PCR negative | 131 | 81 | 39 | 11 |
| Total |
167 |
116 |
40 |
11 |
| Without erythema migrans | ||||
| B. burgdorferi sensu lato | 4 | 4 | 0 | 0 |
| B. miyamotoi | 65 | 55 | 10 | 0 |
| B. burgdorferi sensu lato plus B. miyamotoi | 1 | 0 | 1 | 0 |
| PCR negative | 73 | 73 | 0 | 0 |
| Total | 143 | 132 | 11 | 0 |
*ELISA results are for borrelial IgM or IgG seroconversion. ND, not determined.