Table II.

Leakage of polyols during culture in synthetic medium

Strain	Polyol Content
	−NaCl			+0.6 m NaCl
	Cells	Medium		Cells	Medium
	μmol/L culture medium		%	μmol/L culture medium		%
Glycerol in GPD1	99  ± 4	13,274  ± 287	0.7	309  ± 25	19,497  ± 880	1.6
Sorbitol in S6PDH	99  ± 16	328  ± 16	23.1	166  ± 9	186  ± 3	47.3
Mannitol in MTLD	77  ± 4	218  ± 27	26.0	184  ± 30	156  ± 23	54.2

Yeast cells were cultured in synthetic medium in the absence of NaCl or in the presence of 0.6 m NaCl and collected at an OD₆₀₀ of approximately the amount of polyol in the medium and in cells was determined. Values (%) are the amount of polyol in the cell divided by the total amount of polyol produced. Strains were labeled as in Table I.