Fig. 1.

Setd2 deficiency impairs multiple-hematopoietic compartment development. a qPCR detection of Setd2 expression level in different BM cell subsets, including HSCs (LT-HSC, CD150⁺CD48^-Lin⁻c-Kit⁺Sca1⁺), MPPs (CD48⁺Lin⁻c-Kit⁺Sca1⁺), B cells (B220⁺), T cells (CD3ε⁺), Ery cells (Ter119⁺), GM cells (Gr1⁺Mac1⁺). b, c The absolute number of PB leukocyte and lymphocyte subsets. WT, n = 18; KO, n = 19. d Statistical comparison of the proportions of different lineages between WT and KO BM. n = 4. e Colony-forming unit (CFU) assay of total BM cells. n = 3. f, g Representative FACS analysis (f) and absolute cell count (g) of BM HSPCs from bilateral femurs and tibias. WT, n = 8; KO, n = 9. h CFU assay of BM FLT3⁻-LSKs. n = 3. i In vitro growth curve of BM FLT3⁻-LSK cells. n = 3. All the samples are prepared using mice at 4−5 weeks after pI–pC induction. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001