Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Confocal microscopy visualization of E3 internalization into 22Rv1 prostate cancer cells. Cells were treated for 1 h with 1 μM of either (A) AF488-E3 or AF488-C36 ± CellMask Deep Red Plasma Membrane Stain or (B) AF488-E3 + LysoTracker Deep Red stain to label acidic vesicles. After washing, Hoechst 33342 was added to all samples to stain the nuclei. Fluorescence was observed on a Leica SP5 inverted confocal microscope. (White scale bars: 20 μm.)
