Table 1.

Aggregates containing ≥5,000 producer P. aeruginosa engage in interaggregate signaling

Trap size, pL	Percent QS+, %
	Biomass	Aggregate	Planktonic
2	ND	ND	ND
5	19.2 ± 7.3*	19.3 ± 7.6*	22.2 ± 10.4
10	41.4 ± 8.6†	39.2 ± 8.2†	51.7 ± 12.6†
20	45.25 ± 11.2	44.8 ± 11.1	45.7 ± 10.3

QS⁺ responder total biomass, aggregate biomass and planktonic biomass surrounding traps of increasing size. QS⁺ response was calculated as the total volume of GFP⁺ voxels/total number of mCherry voxels. Data are presented as the mean value of six replicates ± SEM. Analysis by ANOVA following arcsine transformation revealed a significant difference between all datasets for total biomass [F(3, 20) = 14.37 (P ≤ 0.0005)], aggregate biomass [F(3, 20) = 14.18 (P ≤ 0.0005)], and planktonic biomass [F(3, 20) = 9.83 (P ≤ 0.005)].

^*A statistically significant increase in QS⁺ response between 5 and 20 pL traps for total biomass (P = 0.044) and aggregate biomass (P = 0.044).

^†A statistically significant increase in QS⁺ response between 10- and 5-pL traps for total biomass, aggregate biomass, and planktonic biomass (for all three, P = 0.05).