Table 2.
Activity of recombinant CYP9K1 against model substrates and insecticides
Substrate | Specific activity/depletion of parental compound, % | |
−NADPH | +NADPH | |
7-Ethocycoumarin | No activity | 10.6 ± 1.1 |
Deltamethrin | No depletion | 32.0 ± 3.24 |
Bendiocarb | No depletion | No depletion |
Pyriproxyfen | No depletion | 38.0 ± 1.3 |
Activities are given in picomoles of 7-hydroxycoumarin per minute per picomole of P450 for the substrate 7-ethocycoumarin and as percentage depletion of the parental insecticides under assay conditions.