Fig. 4.

In vivo multiplexed CRISPRa using flySAM. (A) Transcriptional activation of hh or ci in the wing (using MS1096-Gal4) results in patterning defects in the anterior and posterior compartments, respectively. The arrow indicates the hh phenotype; the arrowhead, the ci phenotype.) Multiplexed activation of ci and hh produces both individual phenotypes. (B) Multiplexed transcriptional activation of Hippo pathway components in the eye using eyeless-Gal4. (C) Simultaneous CRISPRa of Tsc1+Tsc2 in the eye. In B and C, two or three sgRNAs per target gene were used. (Scale bars: 250 μm.)