Figure 1.

Generation of Scrib conditional knock-out mouse: CaMK-Scrib^−/−. (A) Coronal sections stained with cresyl violet showing normal brain gross anatomy in control and CaMK-Scrib^−/− mice. Scale bar = 1 mm. (B) NeuroTrace 530/615 (red) and DAPI (blue) staining showing expression in the hippocampus of 10-week-old Ai6^f/+/CaMKIIα-Cre/+ double-transgenic mouse (B_a), and a higher magnification of CA1 pyramidal layer (B_b). Scale bar = 40 µm. (C) Scrib expression (green) in dorsal hippocampus of adult Control (C_a) and CaMK-Scrib^−/− (C_b) mice and DAPI staining (blue). Scale bar = 50 µm.

The following figure supplement is available for Figure 1: Supplementary Fig. S1. Generation of Scrib conditional knock-out mouse.