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. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Biochemistry. 2016 Jun 2;55(23):3261–3269. doi: 10.1021/acs.biochem.6b00510

Figure 3.

Figure 3

Spectrophotometric titrations of WT (A) and R174A (B) FDTS with dUMP in 0.1 M Tris-HCl, pH 8 at 25 °C. The insets show the change in absorbance as a function of ligand concentration. The spectral change for dUMP binding to R174A FDTS was noticeably different from other FDTS variants. Fitting to Eq. 1 gives a Kd of 30 nM for dUMP binding to WT FDTS. Fitting to a square hyperbola gives a Kd of 109 μM for dUMP binding to R174A FDTS.