Skip to main content
. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Biochemistry. 2016 Jun 2;55(23):3261–3269. doi: 10.1021/acs.biochem.6b00510

Figure 7.

Figure 7

dU failed to oxidize reduced FDTS in the presence of CH2THF. Anaerobic oxidized FDTS (green) in 0.1 M Tris-HCl, pH 8 at 25 °C was titrated stoichiometrically with dithionite to reduce the flavin (blue). Anaerobic addition of CH2THF perturbed the spectrum, indicating that it formed a complex with the enzyme (black). Anaerobic addition of dU to the reduced FDTS-CH2THF complex perturbed the spectrum further but failed to oxidize the flavin (orange), indicating that the phosphate of dUMP plays an essential role in the FDTS-catalyzed reaction. The flavin could then be re-oxidized by air (red).