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. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Biochemistry. 2016 Jun 2;55(23):3261–3269. doi: 10.1021/acs.biochem.6b00510

Table 1.

Ligand Binding to WT and Variant FDTSs

enzyme + ligand complex Kd (μM)a
WT + dUMP 0.03b ± 0.02
WT + dU 78 ± 5
R90A + dUMP 14 ± 2
R174A + dUMP 109 ± 6
WT + dUMS 0.17b ± 0.08
a

0.1 M Tris-HCl, pH 8 at 25 °C

b

Estimate from fitting to Equation 1. Errors are standard errors on the fits.