. Author manuscript; available in PMC: 2018 May 8.

Published in final edited form as: Biochemistry. 2016 Jun 2;55(23):3261–3269. doi: 10.1021/acs.biochem.6b00510

Table 2.

Chemical Shifts for Uracil Carbons^a

	C2 (ppm)	C4 (ppm)	C5 (ppm)	C6 (ppm)
unbound dUMP pH 8	152.7	167.6	102.8	142.3
unbound dUMP pH 12	159.7	177.2	103.2	140.7
dUMP + WT	160.5	176.4	103.9	141.2
dUMP + R174A	152.6	167.9	103.2	144.4
dUMP + R90A	160.3	177.0	103.7	140.7
dU + WT	160.4	176.6	104.3	138.8

45 °C, pH 8 unless stated otherwise.