. Author manuscript; available in PMC: 2019 Feb 12.

Published in final edited form as: Mol Omics. 2018 Feb 12;14(1):26–36. doi: 10.1039/c7mo00006e

Table 2.

Results from an in-vitro kinase activity assay of a selected panel of pyrrolo[3,4-c]pyrazol compounds from our library against recombinant kinase domains of human and T. brucei GSK3β.

Compound	HsGSK3β IC₅₀ (S.D.) in nM	TbGSK3β IC₅₀ (S.D.) in nM
10	77 (14)^b	360 (100)^b
16	66 (32)^a	5700 (1300)^b
19	77 (18)^c	62 (12)^c
29	170 (1.6)^a	510 (78)^a
18	120 (35)^b	47 (7.7)^b
11	65 (10)^b	200 (34)^b
39	2.8 (0.6)^a	47 (19)^c
38	4.7 (0.8)^c	160 (16)^c

Mean of two replicate experiments,

Mean of three replicate experiments,

Mean of ≥4 replicate experiments.