Figure 1.

Activity of the xylanase proteins. A, β-1-4-Endoxylanase activity of EIX (□) from T. viride and XynI (●) and XynII (▪) from T. reesei. Enzymatic activity was determined as described in “Materials and Methods.” B, Fifty nanograms of EIX, XynI, and XynII proteins was separated on a 15% (w/v) SDS-polyacrylamide gel, transferred to a nitrocellulose filter, and probed with anti-EIX antibodies. C, Ethylene induction was performed with 0.5 μg of each designated protein as described in “Material and Methods.”