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Expression of EIX in sf9 cells. Sf9 cells were infected with baculovirus (lane 1) or baculovirus harboring the Tvx gene (lane 2). Crude lysate and pure EIX (lane 3) proteins were separated on a 15% (w/v) SDS-polyacrylamide gel, transferred to a nitrocellulose filter, and probed with anti-EIX antibodies.
