Figure 6.

Ethylene induction activity of the sf9 expressed EIX proteins. A, Six tobacco leaf discs were treated with EIX (1 μg/mL) or with the mutated proteins (1 μg/mL) for 4 h. Ethylene production was measured by GC as described in “Materials and Methods.” B, Six leaf discs of tobacco were treated with different amounts of EIX (○) or the mutant proteins Gln-86 (□)and Asp-177 (▪) for 4 h. Ethylene production was measured by GC as described in “Materials and Methods.”