Table I.
Biological activity of EIX expressed in sf9 cells
| Elicitor | Ethylene Productiona | Xylanase Activityb |
|---|---|---|
| μL h−1 g−1 | ||
| EIX | 0.475 ± 0.015 | 1.9 ± 0.11 |
| EIX-Bacc | 0.455 ± 0.016 | 2.0 ± 0.05 |
| Control | 0.027 ± 0.005 | 0.02 ± 0.001 |
a
One microgram of EIX protein or 10 μg of sf9 cell lysate was used as a control.
b
Xylanase activity was measured according to the method of Biely et al. (1985). The xylanase activity is shown as OD595. Two-hundred nanograms EIX- or sf9-expressed EIX (the amount of EIX in the sf9 lysate was monitored by immunoblot) was used for the xylanase activity and 1,000 ng of the sf9 cell lysate was used as control.
c
EIX-Bac, Wild-type EIX expressed in sf9 cells by recombinant baculovirus.