Figure 3.

NGA inhibited osteoclast gene expression in mouse BMMs in vitro. The expression of mRNAs corresponding to gene products specific to RANKL-induced osteoclast differentiation, including TRAP, CTR, CTSK, and NFATc1 in mouse BMMs treated with 30 ng/ml M-CSF, 50 ng/ml RANKL, and increasing concentrations of NGA (0, 0.2 or 0.4 μg/ml) for 5 days, measured by real-time PCR. RNA expression levels were normalized relative to the expression of GAPDH. Gene expression after being treated with NGA, compared with the 0 μg/ml treatment group (t-test), * p<0.05 and ** p<0.01, respectively.