Fig. 3. (a) Viability of BMMs with various particle incubation concentrations after 24 and 48 h. (b)–(d) Are slices of 4T1/NIH-3T3 co-cultured breast tumor spheroids imaged by confocal microscopy. Blue is 4′,6-diamidino-2-phenylindole (DAPI) and red represents macrophages stained with CellTracker™ deep red. Green spots in (c) and (d) are Cy-3–IgG labeled microparticles. (b) Spheroids stained with a Hypoxyprobe™ Red549 to label hypoxia (cyan). (c) Spheroid incubated with microparticles-laden BMMs. (d) Spheroid incubated only with IgG modified PLGA microparticles.
