Fig. 6. Top: flow cytometry analysis of dissociated tumor spheroids stained for HIF-1α under different treatments. Gated cells correlate with a positive signal from staining. (i) and (ii) Three runs of analysis for spheroids loaded with PLGA microparticles incorporating I and Nd-UCNPs with and without 735 nm LED irradiation at 0.58 mW cm–2 for 8.5 h. (iii) The comparison of (i) and (ii) for each run. Red and black lines represent particles with and without light irradiation respectively. Bottom: the summary of flow cytometry analysis for the control (i) and the NO release study (ii).
