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. 2018 Jan 9;98(3):309–322. doi: 10.1093/biolre/ioy001

Figure 4.

Figure 4.

Transfected JEG-3 cells express ERAP2 isoforms, and HLA-C expression level is unchanged. ERAP2-transfected JEG-3 cells were used as the stimulator cells in a lymphocyte activation assay. (A) A plasmid employing the RFP and either K392 or N392 ERAP2 protein transfected into JEG-3 cells was employed for lymphocyte activation assays. (B) Western blot analysis shows overexpression of K392 and N392 ERAP2 protein level, which did not produce any change in HLA-C expression. (C) Bar graph of densitometry of transfected JEG-3 cells shows relative protein expression of ERAP2 and HLA-C. One-way ANOVA of ERAP2 expression of JEG-3(K) and JEG-3(N) indicates a significant difference compared to untransfected JEG-3 cells (* and # P < 0.05, n = 3). There were no statistically significant differences in HLA-C expression among experimental groups JEG-3(K) or JEG-3(N) compared to the control group of JEG-3 cells without transfection (P > 0.05, n = 3).