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. 2018 Feb 7;159(4):1595–1608. doi: 10.1210/en.2017-03250

Figure 2.

Figure 2.

BPA ex vivo treatment activates PXR in the heart tubes of PXR-humanized embryos. (A) Scheme of animal models and embryo genotypes. Female PXR−/−ApoE−/− mice were mated with male huPXR•ApoE−/− mice. The embryos at E9.5 were isolated and cultured in control media or media supplemented with 20 μM BPA for 24 hours. (B) Representative images of cultured embryos. Scale bar = 2.5 mm. (C) The heart tubes of the embryos were dissected for RNA extraction, and messenger RNA (mRNA) levels of PXR target genes were analyzed using qPCR (n = 4 to 7; **P < 0.01).