Figure 1.

GLP1R mRNA transcript levels in the human heart are comparable to those in human pancreas and islets. (A) GLP1R mRNA levels were measured via qPCR analysis in multiple human tissues and in transfected BHK cells that express low levels of the human GLP-1R. For data represented without standard error bars, each single RNA sample was analyzed in duplicate; for isolates depicted with error bars, peripheral blood lymphocyte samples were analyzed in duplicate from two different sources, and at least three different samples were analyzed in duplicate by qPCR for RNAs from islet, bone marrow, left atria (LA), right atria (RA), left ventricle (LV), and right ventricle (RV). (B) qPCR analysis of GLP1R and tissue- or cell-type–specific gene expression in the indicated samples as confirmation of RNA/cDNA integrity. For (A) and (B), data are expressed as cycle threshold (Ct) values because none of the housekeeping genes examined (ACTB, GPI, PSMB4, CHMP2A, and EMC7) exhibited consistent expression levels in all tissues examined. Values are mean ± standard error (where appropriate); n = 1 to 3 samples per tissue. LA samples are from patients P01371, P01430, and P01504. RA samples are from patients P01262, P01371, and P01377. LV samples are from patients P01262, P01430, and P01371. RV samples are from patients P01262, P01371, and P01504 (see Supplemental Table 1 and Figure 4). Islet samples are from donors R177, R199, and R200 (see Supplemental Table 3). CA EC, coronary artery endothelial cells; CA SMC, coronary artery smooth muscle cells; PBL, peripheral blood lymphocytes; Card FB, cardiac fibroblasts.