Fig. 4. (a) siRNA cell uptake efficiency. Fluorescence positive cell (%) was measured using flow cytometry. HeLa cells were incubated with siRNA/stapled peptide complexes formed with a 1 : 10 molar ratio (50 nM of siGLO, mean ± s.d., n = 3). (b) Relationship between the siRNA cell uptake and complex formation efficiency at 1 : 10 molar ratio. The dotted line is a trend line. R2 (squared correlation coefficient) and the linear regression formula are shown. (c) Relative mRNA expression of the target gene transfected using siRNA/other stapled peptide complexes formed with a 1 : 10 molar ratio (50 nM of siRNA). HeLa cells were transfected and incubated for 24 h (mean ± s.d., n = 3). (d) Relationship between siRNA cell uptake and in vitro gene silencing efficiency of siRNA-peptide complexes in HeLa cells. Data for LKacr-stEK and stEK and those for a group showing gene silencing efficiency with over 50% are indicated in a gray circle and a red box, respectively.
