Spatial and temporal expression of Cv20ox. A, The blot of the RNAs isolated from different parts of the mature plant was hybridized with the same probe as that used in genomic DNA-blot analysis. I, Integuments (8 DAP); S, whole seeds (8 DAP); IS, inner seed tissues (8 DAP); F1, fruits (1 DAP); F2, fruits (2–3 DAP); F8, fruits (8 DAP); T, tendrils; L, leaves; M, male flowers; O, ovaries before pollination. B, RNAs isolated from different organs of young plants grown for 15 d after imbibition were blotted and hybridized with the same probe as that described above. C, Cotyledons; Sm, shoot meristem including uppermost of hypocotyls; Hu, upper hypocotyls; Hl, lower hypocotyls; R, roots. Twenty-five micrograms of RNA was used for the blot analyses. C, Temporal expression pattern of Cv20ox during seed development. Twenty micrograms of RNAs isolated from whole seeds at seven different stages after pollination was resolved on a 0.8% agarose gel, transferred onto a nylon membrane, and hybridized with the Cv20ox probe.