Figure 5.

Localization of the Cv20ox mRNA in watermelon seeds by in situ hybridization. All samples were sectioned longitudinally in parallel with the plane of seed. Micrographs show pollinated seeds at 5 DAP (A, B, and C), at 6 DAP (D and E), and at 4 DAP (F and G). The sections were hybridized to either an antisense (B, E, and G) or a sense (C) RNA probe of the entire cDNA that was labeled with digoxigenin. A, D, and F are toluidine-blue-stained samples. nu, Nucellus; ii, inner layer of integument; oi, outer layers of integument; mp, micropylar end; e, globular embryo. Bar = 100 μm.