| Recombinant protein arrays |
Content spans nearly the entire complement of proteins encoded in the genome |
Not suited for epitopes associated with posttranslational modifications |
| Natural protein arrays |
Well suited for epitopes associated with posttranslational modifications |
Require isolation from tumor sources and verification of antigen identity |
| Peptide arrays |
Scope covers the entire complement of peptides encoded in the genome |
Limited to linear epitopes |
| Mass spectrometry identification of antigen bound to Ig or soluble HLA molecules |
Allows detection of bound antigen |
Complexes may be cleared from the circulation and thus not detectable |
