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Wild-type and mutated xylanase II expressed in yeast cells. SDS-PAGE of proteins from culture medium of non-transformed yeast (Y294), and yeast expressing wild-type (WT) and mutated (E210S and E210D) forms of xylanase II. Lanes were loaded with 20 μg of total protein present in cell supernatants (S) or with 5 μg of protein after purification by cation-exchange chromatography. Standard molecular mass markers are indicated on the left.
