Alkalinization response and induction of ethylene biosynthesis in suspension-cultured tomato cells. A, Extracellular pH in tomato cells treated for 15 min with different doses of wild-type (●), mutant E210S (▾), and mutant E210D (□) xylanase II. Symbols represent the average of three measurements and bars represent sd. B, Induction of ethylene biosynthesis in tomato cells by different doses of wild-type (●), mutant E210S (▾), and mutant E210D (□) xylanase II. Ethylene accumulating in the free-air phase of the cultures was measured 4 h after of treatment. Symbols represent the average of three measurements and bars represent sd.