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Induction of necrosis in tomato and tobacco leaves. Tomato (A) and tobacco (B) leaves injected with wild-type (WT) and mutant forms (E210S and E210D) of xylanase II and water as a control. Leaves were injected with xylanase preparations at a concentration of 1 mg/mL for tomato leaves and 10 μg/mL for tobacco leaves. C, Tobacco leaf injected with different concentrations of wild-type and mutant E210S xylanase as indicated.
