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. 2018 Apr 17;10(4):632–644. doi: 10.18632/aging.101418

Figure 5.

Figure 5

KCNQ1OT1 promotes melanoma cell proliferation. (A) After transfecting cells with KCNQ1OT1, proliferation rates of A375 and A875 were determined using MTT assays. Data are presented as means ± SEM. (B) After transfecting cells with si-KCNQ1OT1, the proliferation rates of A375 and A875 were determined using MTT assays. Data are presented as means ± SEM. (C, D) After transfecting KCNQ1OT1/control in A375 cells, the cyclin d1 protein and mRNA level was detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P< 0.01 (E, F) After transfecting si-KCNQ1OT1 or control in A375 cells, cyclin d1 protein and mRNA level was detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P< 0.01.