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. 2018 Apr 17;10(4):632–644. doi: 10.18632/aging.101418

Figure 7.

Figure 7

miR-153 mediates the effect of KCNQ1OT1 on proliferation and metastasis in melanoma cells. (A) After transfecting with control, miR-153, or KCNQ1OT1 and miR-153, A375 and A875 cell proliferation was detected using an MTT assay. Data are shown as mean ± SEM. (B) After transfecting control, miR-153, or KCNQ1OT1 and miR-153 in A375 cells, a transwell assay without matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. ** P < 0.01 (C, D) Western blot and real time PCR was used to detect indicant proteins in A375 cells. ** P < 0.01 (E) After transfecting with control, AS-miR-153, or si-KCNQ1OT1 and miR-153 AS, A375 and A875 cell proliferation was detected using an MTT assay. Data are shown as mean ± SEM. (F) After transfecting control, ASO-miR-153, or si-KCNQ1OT1 and miR-153 AS in A375 cells, a transwell assay without matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. ** P< 0.01 (G, H) Western blot and real time PCR were used to detect indicant proteins in A375 cells. ** P< 0.01.