Table 4. Transfection efficiency PCR primers.
Name | Forward primer (5'->3') | Reverse primer (5'->3') | |
KCNQ1OT1 | TTCACGCACATGTAGGCAC | CAAGCTCCACTCCTCACCTG | |
si-KCNQ1OT1 | CAGGTGAGGAGTGGAGCTT | GTGCCTACATGTGCGTC | |
KCNQ1OT1 MUT | GACACACATGGCTCAGGACA | GTGCTGTCCCCACACTTTCT | |
MET WT | CGACAGCTGACTTGCTGAG | GGTTTATCTTTCGGTGCCC | |
MET MUT | GAGCGCTTTGTGAGCAGAT | GGTTTATCTTTCGGTGCCC | |
MiR-153 | CTCACAGCTGCCAGTGTCATTT | CACTTTTGTGACTATGCAACTGG | |
control | UUUGUACUACACAAAAGUACUG | ||
miR-153 AS | GTTGCATAGTCACAAAAGTG | AATTGACACTGGCAGCTGTG | |
Anti-sense control | UCACAACCUCCUAGAAAGAGUAGA |