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. 2018 Apr 25;10(4):747–763. doi: 10.18632/aging.101425

Figure 3.

Figure 3

Fibrogenic differentiation ability was diminished in PMS-2G11 cells. (A) Quantification of mRNA levels of fibrosis-related markers in 2G11 and PMS-2G11 cells treated with or without TGFβ1. CTGF: connective-tissue growth factor; Col1a1: collagen type 1; Acta2: α-actin-2. Data are expressed as means±SE (n=3); distinct letters (a–d) indicate statistically significant differences (P<0.05). (B) Immunocytochemical analysis of α-smooth muscle actin expression in 2G11 and PMS-2G11 cells treated with or without TGFβ1. Arrowheads: stress fibres. Scale bar: 100 μm. (C) Immunoblotting analysis of α-smooth muscle actin expression in 2G11 and PMS-2G11 cells treated with or without TGFβ1. (D) Quantification of α-smooth muscle actin protein expression. Data are expressed as means±SE (n=3); distinct letters (a, b) indicate statistically significant differences (P<0.05).