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. 2018 Mar 9;8(5):1555–1569. doi: 10.1534/g3.117.300571

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Copyright © 2018 Das et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Conserved domains in the N-terminus are dispensable for meiosis but regulate ectopic microtubule bundling in ooplasm. Oocytes stained for DNA (blue), HA (red), tubulin (green). Single channels for HA and tubulin are shown in white. Scale bars are 5 µm. A, B. Localization of Subito^Δ(24-33) in sub⁺ or sub¹³¹^/¹background. Subito^Δ(24-33) shows normal localization. C, D. Localization of Subito^S16AS24A in sub⁺ or sub¹³¹^/¹background. (n= 21, 5, 22 and 5 respectively for panels A-D). E, F. Examples of ectopic microtubule bundling in oocytes expressing Subito^S16AS24A although it is not as severe as Subito^ΔNT.