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. 2018 Mar 9;8(5):1555–1569. doi: 10.1534/g3.117.300571

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Copyright © 2018 Das et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The poorly conserved domain 42-76 is required for establishing bipolarity and homolog bi-orientation. A,B. Localization of Subito^Δ(42-76) in sub⁺ or sub^131/1 null background oocytes. Oocytes are stained for DNA (blue), HA (red), tubulin (green). Single channels for HA and tubulin are shown in white. Scale bars are 5 µm. C,D. FISH in the indicated genotypes using probes for the X (359 in magenta), 2^nd (AACAC in red) and 3^rd chromosomes (Dodeca satellite in white). (n= 28, 17, 18 and 12 respectively).