Figure 4.

Using BE3 to knock out the endogenous GAPDH and V-ATPase B genes by introducing a premature stop codon. (A) Protein levels were analyzed by western blotting at 12 days post-transfection. Alpha-tubulin was detected as a control protein. (B) Sanger sequencing of genomic regions targeted by GAPDH and V-ATPase B gRNAs. The encoded amino acids are shown below. Asterisks in red represent stop codons. (C) The number of genes with different numbers of targetable knockout sites per gene. (D) Relative positions of knockout sites in CDSs. Four targetable codons are shown in different colors.