Fig 5. rSpTrf-E1 has binding specificity to LPS.

Increasing concentrations of rSpTrf-E1 pre-bound to anti-SpTrf were analyzed by ELISA. In the absence of the antisera, significantly more rSpTrf-E1 is bound to LPS compared to the background controls in the absence of LPS. Pre-incubation of rSpTrf-E1 with anti-SpTrf antibodies decreases binding to LPS significantly compared to samples in which the antisera are omitted. Data are shown as the mean and ± standard deviation (SD) of three replicates. Brackets indicate significance (p < 0.05) using Student’s two-tailed paired t test with equal variance.