Fig 6. cNi-SpTrf proteins are not contaminated with detectible amounts of SpC3.

Pelleted coelomocytes were sonicated, the lysate was passed through a nickel column, and an eluted fraction containing cNi-SpTrf proteins was evaluated by Western blot with anti-SpC3-6His [64]. A sample of cfCF that was not subject to nickel affinity was evaluated on the same filter for comparison. The cNi-SpTrf proteins do not contain detectable traces of SpC3 while the cfCF fraction has an autolytic fragment of the SpC3 α chain [70] indicating the presence of an active complement protein homologue.