Table 2. Stability rankings of candidate reference genes calculated by geNorm, NormFinder, and BestKeeper algorithms.

Comprehensive ranking of candidate reference genes using RefFinder.

	Rank	geNorm	NormFinder	BestKeeper	RefFinder
Two-Row Barley	1	ADP | α-Tub	HSP70	TCPT	HSP70
	2		SnoR14	ADP	ADP
	3	ACT	ACT	HSP70	α-Tub
	4	GRBP	ADP	α-Tub	ACT
	5	HSP90	UBQ	SnoR14	SnoR14
	6	TCPT	GRBP	SnoR23	TCPT
	7	HSP70	α-Tub	HSP90	GRBP
	8	SnoR14	TCPT	UBQ	UBQ
	9	UBQ	HSP90	ACT	HSP90
	10	ABC	ABC	GRBP	SnoR23
	11	SnoR23	SnoR23	ABC	ABC
	12	GAPDH	GAPDH	GAPDH	GAPDH
	13	CYP	CYP	CYP	CYP
Six-Row Barley	1	ACT | GRBP	ACT	UBQ	ACT
	2		GRBP	GAPDH	GRBP
	3	ADP	HSP70	SnoR14	UBQ
	4	α-TUB	UBQ	TCPT	HSP70
	5	HSP70	ADP	HSP70	ADP
	6	UBQ	α-TUB	ACT	SnoR14
	7	HSP90	SnoR14	SnoR23	α-TUB
	8	TCPT	TCPT	ADP	TCPT
	9	SnoR14	HSP90	GRBP	GAPDH
	10	SnoR23	GAPDH	α-TUB	HSP90
	11	GAPDH	SnoR23	HSP90	SnoR23
	12	ABC	ABC	ABC	ABC
	13	CYP	CYP	CYP	CYP
All samplesa	1	ACT | GRBP	HSP70	TCPT	ACT
	2		ACT	UBQ	HSP70
	3	ADP	GRBP	SnoR14	GRBP
	4	α-TUB	UBQ	SnoR23	UBQ
	5	HSP90	ADP	HSP70	ADP
	6	HSP70	SnoR14	ADP	TCPT
	7	UBQ	α-TUB	ACT	SnoR14
	8	SnoR14	TCPT	GAPDH	α-TUB
	9	TCPT	HSP90	GRBP	SnoR23
	10	SnoR23	SnoR23	α-TUB	HSP90
	11	ABC	ABC	HSP90	GAPDH
	12	GAPDH	GAPDH	ABC	ABC
	13	CYP	CYP	CYP	CYP

^a All samples: Two- and six-row samples combined.